Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Half tetrad analysis in alfalfa using multiple restriction fragment length polymorphism markers

S Tavoletti1, E T Bingham, B S Yandell

  • 1Department of Agronomy, University of Wisconsin, Madison 53706, USA.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1996
PubMed
Summary

A new half tetrad analysis (HTA) method uses restriction fragment length polymorphism (RFLP) markers to study 2n gamete formation. This approach accurately determined that alfalfa produced mostly second division restitution 2n eggs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unlocking diagnosis of sarcopenia: The role of circulating biomarkers - A clinical systematic review.

Mechanisms of ageing and development·2024
Same author

Toxoplasma gondii and Neospora caninum infection in epileptic dogs.

The Journal of small animal practice·2024
Same author

A Pleasant Remedy for Toothache.

The Independent practitioner·2023
Same author

The Intimate Connection between Areas of Low Barometer and the Prevalence of Epidemics of Non-Contagious Diseases.

Daniel's Texas medical journal·2023
Same author

Johnson County Medical Society.

Daniel's Texas medical journal·2023
Same author

Screw Worms in the Nose: Reply to Dr. Gilbert's Request in November Number.

Daniel's Texas medical journal·2023

Area of Science:

  • Plant genetics
  • Reproductive biology
  • Molecular markers

Background:

  • Understanding 2n gamete formation is crucial for plant breeding and crop evolution.
  • Existing methods for analyzing gamete formation can be limited in scope and precision.

Purpose of the Study:

  • To develop a novel maximum likelihood approach for half tetrad analysis (HTA).
  • To estimate the frequencies of different 2n gamete formation mechanisms (FDR and SDR).
  • To simultaneously locate centromeres within RFLP linkage groups.

Main Methods:

  • Developed a maximum likelihood-based HTA using multiple RFLP markers.
  • Applied the method to a diploid alfalfa clone (PG-F9) heterozygous for codominant RFLP alleles.
  • Utilized models with linked and unlinked RFLP loci to analyze 2n egg production.

Related Experiment Videos

Main Results:

  • Alfalfa clone PG-F9 produced 6% first division restitution (FDR) and 94% second division restitution (SDR) 2n eggs.
  • Successfully located centromeres on linkage groups using marker locus information.
  • HTA results align with cytological data, suggesting diplospory as the FDR mechanism in PG-F9.

Conclusions:

  • Multilocus HTA is an effective tool for analyzing 2n gamete formation and centromere mapping.
  • The findings provide insights into the reproductive biology of alfalfa and apomictic species.
  • This method has potential applications in studying plant reproductive mutants and advancing crop breeding.