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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Transcriptional regulatory elements in the rat bradykinin B2 receptor gene
J B Pesquero1, C J Lindsey, A C Paiva
1Department of Biophysics, Escola Paulista de Medicina, Säo Paulo, Brazil.
Abstract:
In an attempt to elucidate the mechanisms underlying the regulation of bradykinin B2 receptor gene expression, the molecular structure of the rat gene including the 5'-flanking region was characterized (J. Biol. Chem. 269: 26920-26925, 1994). In this study we show that the gene spans about 32 kb, including a long first intron of 25 kb. The promoter region drives reporter gene expression in NG108-15 neuroglioma cells, and its expression is upregulated by cAMP, bradykinin, phorbol esters and by coexpression of an activated ras oncogene but not by dexamethasone.
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