Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sensing stress and responding to stress

R Voellmy1

  • 1Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, FL 33101, USA.

EXS
|January 1, 1996
PubMed
Summary

Proteotoxic stress increases heat shock protein gene expression via heat shock transcription factor 1 (HSF1). The precise mechanism sensing stress and activating HSF1 remains under investigation, with current models involving heat shock protein 70.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat Shock Protein Induction in Montastraea faveolata and Aiptasia pallida Exposed to Elevated Temperatures.

The Biological bulletin·2017
Same author

Feedback regulation of the heat shock response.

Handbook of experimental pharmacology·2006
Same author

Induction of cell stress through gene transfer of an engineered heat shock transcription factor enhances tumor immunogenicity.

Gene therapy·2004
Same author

Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex.

The Journal of biological chemistry·2001
Same author

Geldanamycin restores a defective heat shock response in vivo.

The Journal of biological chemistry·2001
Same author

A transcriptional feedback loop for tissue-specific expression of highly cytotoxic genes which incorporates an immunostimulatory component.

Gene therapy·2001

Area of Science:

  • Molecular biology
  • Cellular stress response
  • Gene regulation

Background:

  • Proteotoxic stress, conditions favoring protein unfolding, enhances heat shock protein gene expression.
  • This upregulation is primarily mediated by the heat shock transcription factor 1 (HSF1).

Purpose of the Study:

  • To elucidate the mechanism by which adverse conditions trigger HSF1 activation.
  • To discuss current models of HSF1 regulation, including the role of heat shock protein 70.

Main Methods:

  • Discussion of existing models and literature.
  • Analysis of the interplay between stress, HSF1, and heat shock proteins.

Main Results:

  • HSF1 oligomerization and DNA-binding ability are induced by stress.
  • Further conformational changes render the DNA-bound HSF1 transcriptionally active.
  • Heat shock protein 70 is implicated as a stress sensor and negative regulator of HSF1.

Conclusions:

  • The precise sensing mechanism for proteotoxic stress leading to HSF1 activation is not fully understood.
  • Alternative models involving additional protein factors in HSF1 regulation are considered.

Related Experiment Videos