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Related Experiment Videos

Starvation-induced multiresistance in Enterococcus faecalis JH2-2

J C Giard1, A Hartke, S Flahaut

  • 1Laboratoire de Microbiologie de l'Environnement, Universite de Caen, France.

Current Microbiology
|May 1, 1996
PubMed
Summary

Carbohydrate starvation induces multiresistance in Enterococcus faecalis cells against various stresses like heat and acid. This resistance involves specific protein synthesis and potentially protein degradation, but not against UV irradiation.

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Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Stress Response

Background:

  • Growing bacteria exhibit susceptibility to various environmental stressors.
  • Understanding bacterial adaptation mechanisms is crucial for controlling infections and developing novel therapies.

Purpose of the Study:

  • To investigate the development of a multiresistance state in carbohydrate-starved Enterococcus faecalis cells.
  • To elucidate the kinetics and underlying mechanisms of stress resistance acquisition.

Main Methods:

  • Enterococcus faecalis cells were subjected to carbohydrate starvation.
  • Resistance to heat, hydrogen peroxide (H2O2), acid, ethanol, and UV irradiation was assessed.
  • Protein synthesis inhibition and antibiotic treatments were employed.

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  • Two-dimensional (2-D) gel electrophoresis was used to analyze protein profiles.

    • Main Results:

    • Carbohydrate-starved cells developed multiresistance to heat, H2O2, acid, and ethanol, but not UV irradiation.
    • Resistance acquisition kinetics varied with stress type; ethanol resistance peaked at 3 hours, while others increased progressively.
    • Protein synthesis inhibition partially impaired heat and oxidative resistance acquisition.
    • Specific proteins were synthesized during early starvation, suggesting a role in resistance.

    Conclusions:

    • Carbohydrate starvation induces a complex multiresistance phenotype in Enterococcus faecalis.
    • Acquisition of maximal resistance to heat and oxidative stress requires early protein synthesis followed by mechanisms involving protein degradation or alteration.