Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of a replication-terminator protein complexed with DNA

K Kamada1, T Horiuchi, K Ohsumi

  • 1Department of Genetics, The Graduate University for Advanced Studies, and National Institute of Genetics, Shizuoka, Japan.

Nature
|October 17, 1996
PubMed
Summary

The Escherichia coli Tus protein

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes of linezolid-related adverse events in patients with multidrug-resistant TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
Same author

Epileptiform Discharges and High-Frequency Oscillations (HFOs) Aid in Identifying Seizure Location.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Myeloperoxidase-anti-neutrophil cytoplasmic antibody positivity and disease characteristics, treatment, and prognosis in eosinophilic granulomatosis with polyangiitis.

Scandinavian journal of rheumatology·2025
Same author

Evaluation of testing face-mask filter samples with LAMP shows high rates of detection in pulmonary TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2024
Same author

Growth of bulk β-Ga<sub>2</sub>O<sub>3</sub> crystals from melt without precious-metal crucible by pulling from a cold container.

Scientific reports·2024
Same author

Evaluation of anatomical references for locating the course of the posterior superior alveolar artery for dental implant surgery.

International journal of oral and maxillofacial surgery·2021

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • DNA replication termination is crucial for genome stability.
  • The Tus-Ter complex is essential for preventing replication fork collisions in bacteria.

Purpose of the Study:

  • To elucidate the structural basis of Tus-Ter DNA complex formation.
  • To understand the mechanism of directional replication fork arrest.

Main Methods:

  • X-ray crystallography
  • Protein-DNA complex analysis
  • Structural determination at 2.7 A resolution

Main Results:

  • The crystal structure of the Tus-Ter complex was determined.
  • Tus protein exhibits a novel two-domain architecture with a central cleft.
  • The complex interfaces with deformed Ter DNA via major groove interactions.

Conclusions:

  • The unique Tus protein structure facilitates specific binding to Ter DNA.
  • Structural insights explain the unidirectional halting of DNA replication forks.

Related Experiment Videos