Nervus terminalis projection to the retina in the 'four-eyed' fish, Anableps anableps

The eye of the surface dwelling 'four-eyed' fish, Anableps possesses an aquatic and an aerial optical system. The aerial system is strongly hyperopic when the animal dives, i.e. during mating, and the dorsal pupil is submerged. We studied the retino-petal nervus terminals projection to the aerial and to the aquatic retina by Phe-Met-Arg-Phe-NH2 (FMRF) immunocytochemistry and found both to be equally innervated. This finding sheds doubt on the proposed functional significance of this projection for reproductive behaviour.