Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple forms of staphylococcal alpha-toxin

A B Dalen

    Acta Pathologica Et Microbiologica Scandinavica. Supplement
    |December 1, 1975
    PubMed
    Summary

    Researchers purified staphylococcal alpha-toxin using various methods. Heat activation yielded pure alpha-toxin, revealing its association with other proteins in a complex.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Prevalence of GB virus C (also called hepatitis G virus) markers in Norwegian blood donors.

    Journal of clinical microbiology·2000
    Same author

    Polyclonal activation of B-lymphocytes and induction of autoimmunity in retrovirus infected NMRI mice.

    Scandinavian journal of immunology·1993
    Same author

    A low oncogenic variant of Friend murine leukemia virus with strong immunosuppressive properties.

    Archives of virology·1993
    Same author

    Isolation of an acidic polysaccharide antigen from Propionibacterium acnes.

    Archives of dermatological research·1985
    Same author

    Rabbit antibodies against the major internal protein of a retrovirus-like particle bind to epidermal cells in psoriatic skin. Brief report.

    Archives of virology·1985
    Same author

    The major internal protein, p27, of a retrovirus-like particle is expressed in blood lymphocytes from psoriatic patients.

    Archives of virology·1985

    Area of Science:

    • Microbiology
    • Protein Biochemistry

    Background:

    • Staphylococcal alpha-toxin is a key virulence factor.
    • Understanding its purification and properties is crucial for studying staphylococcal infections.

    Purpose of the Study:

    • To investigate methods for extracting and purifying staphylococcal alpha-toxin.
    • To characterize the purified alpha-toxin and its associated proteins.

    Main Methods:

    • Extraction of proteins from staphylococcal culture filtrate precipitates.
    • Activation of alpha-toxin using urea, acidic buffers, or heat.
    • Purification using hydroxyl apatite chromatography and isoelectric focusing.
    • Analysis of protein components using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Sephadex G-200 chromatography.

    Main Results:

    • Proteins were extracted at neutrality, while alpha-toxin required activation.
    • Heat activation yielded relatively pure alpha-toxin (39,000 molecular weight).
    • Alpha-toxin co-eluted with three other proteins, suggesting complex formation.
    • Isoelectric focusing identified hemolytic fractions, with some containing alpha-toxin and other proteins.

    Conclusions:

    • Staphylococcal alpha-toxin can be purified through specific activation and chromatographic techniques.
    • Alpha-toxin exists in association with other proteins, forming a high molecular weight complex.
    • Further characterization of this complex may provide insights into staphylococcal toxin function.

    Related Experiment Videos