Related Experiment Videos
Partial purification and characterization of Salmonella cytotoxin
P Malik1, V D Sharma, D C Thapliyal
1Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, India.
Abstract:
The cytotoxins of two strains of Salmonella enterica subspecies enterica serovar Nchanga (MP2309) and Virchow (MP1871) were partially purified and characterized physicochemically. The toxin was salt-precipitable and non-dialysable. Upon filtration through Sephadex G-100 gel column, the toxicity was observed mainly in 2nd peak. The cytotoxic activity was lost completely when heated at 121 degrees C for 15 min. The toxin was found active between a narrow range of pH (i.e., 6.0 to 9.0). The cytotoxic activity was destroyed by treatment with trypsin and protease but the activity was not affected by papain.