Seroepidemiology of rickettsial infections in Morocco

The prevalence of antibodies reactive with Rickettsia conorii, Rickettsia typhi, Coxiella burnetii and Ehrlichia chaffeensis was investigated using indirect immunofluorescence (IFA) test on human sera obtained from 300 blood donors in Casablanca and 126 sera obtained from clinical laboratories in Fez. In sera from Casablanca, antibodies reactive at titers > or = 1:32 were found against R. conorii (7%), and R. typhi (1.7%), but not against E. chaffeensis. In the sera from Fez, antibodies were also detected against R. conorii (5.6%), R. typhi (4%), but not against E. chaffeensis. By Western immunoblotting, seroprevalence for R. conorii was in Casablanca and 4.8% in Fez. Antibodies reactive at titers > or = 1:50 against C. burnetii (phase II) were present in sera from Casablanca (1%) and Fez (18.3%).