Related Experiment Video
Updated: May 8, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
Published on: August 22, 2010
Interaction between the origin recognition complex and the replication licensing system in Xenopus
1Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, United Kingdom.
Abstract:
The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.
Related Concept Videos
Types of Genetic Transfer Between Organisms
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Types of Genetic Transfer Between Organisms

