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Sub-typing of animal and human Campylobacter spp. using RAPD
R H Madden1, L Moran, P Scates
1Food Science Department, Department of Agriculture for Northern Ireland, Belfast, UK. R.Madden@QUB.AC.UK
Letters in Applied Microbiology
|September 1, 1996
Abstract:
Based on a 10-mer primer (5'-CCTGTTAGCC-3'), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. The method was also successfully applied to typing Campylobacter jejuni from a similar range of sources.