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Updated: Aug 9, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Detection of Escherichia coli O157:H7 in water from coliform enrichment cultures
E W Rice1, C H Johnson, D J Reasoner
1US Environmental Protection Agency, Cincinnati, OH 45268, USA.
Abstract:
Environmental water samples were seeded with Escherichia coli O157:H7 and the bacterium was recovered using a traditional coliform enrichment procedure followed by selective plating on sorbitol MacConkey agar and biochemical and serological characterization. Assays for beta-glucuronidase and glutamate decarboxylase were found to be useful procedures for screening suspected isolates. The organism was not recovered in a survey of various water samples.
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