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Related Experiment Videos

Human sperm coating antigen from seminal plasma origin

A Iborra1, C Morte, P Fuentes

  • 1Unitat d'Immunologia, Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.

American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|August 1, 1996
PubMed
Summary

A novel monoclonal antibody (SEM-12) targets human sperm glycoproteins, inhibiting motility and acrosome reaction. This antibody identifies an 80-84 kDa antigen present on sperm surfaces and in seminal plasma across species.

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Area of Science:

  • Reproductive Biology
  • Immunology
  • Biochemistry

Background:

  • Sperm surface glycoproteins play a crucial role in sperm-zona pellucida recognition during fertilization.
  • Seminal plasma proteins can influence sperm function, with their expression potentially altering during capacitation and acrosome reaction.
  • Sperm-specific monoclonal antibodies are vital tools for identifying and characterizing sperm membrane-associated proteins and their functions.

Purpose of the Study:

  • To isolate and characterize a novel monoclonal antibody (SEM-12) specific for human sperm.
  • To investigate the distribution and function of the antigen recognized by SEM-12.
  • To determine the role of this antigen in sperm function, including motility and acrosome reaction.

Main Methods:

  • Indirect immunofluorescence was used to determine the antigen distribution on human sperm.

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  • Enzyme-linked immunosorbent assay (ELISA) was employed to detect the antigen in seminal plasma.
  • Affinity chromatography and immunoprecipitation were utilized to isolate and identify the proteins recognized by SEM-12.
  • The effect of SEM-12 on sperm motility and acrosome reaction was assessed.

    • Main Results:

    • The SEM-12 antibody demonstrated a discontinuous distribution of its target antigen on the head and tail surfaces of non-capacitated human sperm.
    • The antigen was detected in human seminal plasma and in the sperm of other species (goat, ram, mouse).
    • Two proteins, ranging from 80-84 kDa, were isolated using SEM-12 via affinity chromatography and immunoprecipitation.
    • SEM-12 significantly inhibited both spontaneous and ionophore-induced sperm motility and acrosome reaction.

    Conclusions:

    • The SEM-12 antibody recognizes a specific 80-84 kDa sperm-associated antigen with potential involvement in sperm-zona pellucida interactions.
    • This antigen, present in seminal plasma and on sperm across species, plays a critical role in regulating sperm motility and the acrosome reaction.
    • SEM-12 serves as a valuable tool for studying sperm function and may have implications for understanding male fertility and developing novel contraceptive strategies.