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Neutrophil activation and chemotaxis after in vitro treatment with perfluorocarbon
J E Rossman1, M G Caty, G A Rich
1Department of Pediatric Surgery, Children's Hospital, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, NY, USA.
Abstract:
The in vitro effects of perfluorocarbon (PFC) on human neutrophil activation and chemotaxis were examined. Neutrophils were incubated, with and without PFC, and were analyzed for chemotaxis through 5-microns and 8-microns pore filters. Neutrophil activation was quantitated by flow cytometry. Activation studies showed that PFC-treated neutrophil samples (n = 6) produced only 39.83% +/- 25.9% (mean +/- SD) of matched control (n = 6) fluorescence. Chemotaxis studies showed that PFC-treated neutrophil samples (n = 8) had migration of only 18.63% +/- 6.5% (of control values) of neutrophils to the outer boundary of the 5-microns filters (n = 8 controls). The 8-microns pore filter migration (n = 8) was similarly low, with a mean outer boundary migration count of only 26% +/- 19.8% of the control (n = 8) value. Thus, neutrophils exposed to perfluorocarbon produce significantly less detectable H2O2 (P < .001) and have a significantly lower chemotactic response (P < .001).