Determination of the steady state tissue distribution of midazolam in the rat

The aim of this study was to determine the steady state tissue:blood (Kb), tissue:plasma (Kp), and tissue:plasma water (Ku) partition coefficients of midazolam in all major organs and tissues in the rat, after development of a gas chromatographic assay. The Kp in 12 tissues ranged between 1.3 (in muscle) and 9.0 (in fat). Ku ranged correspondingly between 16 and 115.