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Updated: Jul 31, 2026

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
Ureaplasma gallorale, an isolate from chickens, is most closely related to the human isolate, U. urealyticum
1Department of Biological Sciences, University of Alberta, Edmonton, Canada. gw_stemke@biology.ualberta.ca
Abstract:
Ureaplasma gallorale is a urease-containing mycoplasma (a member of the Mollicutes) which is pathogenic for chickens, from which it was originally isolated. We amplified the 16S rRNA gene of this bacterium and then cloned and sequenced the amplicon. A phylogenetic analysis based on an alignment of the 16S rRNA sequences of U. gallorale and several other Ureaplasma species revealed that U. gallorale is more closely related to Ureaplasma urealyticum than to other Ureaplasma species.
Insights
Ureaplasma gallorale, a chicken pathogen, was analyzed using its 16S rRNA gene. Phylogenetic analysis shows it is most closely related to Ureaplasma urealyticum.
Area of Science:
- Veterinary Microbiology
- Bacterial Phylogenetics
- Mollicutes Research
Background:
- Ureaplasma gallorale is a urease-containing mycoplasma identified as pathogenic in chickens.
- Mycoplasmas, belonging to the class Mollicutes, are known for their diverse host associations and pathogenic potential.
Purpose of the Study:
- To determine the phylogenetic position of Ureaplasma gallorale within the Ureaplasma genus.
- To clarify the evolutionary relationships of U. gallorale with other related species.
Main Methods:
- Amplification of the 16S rRNA gene from Ureaplasma gallorale.
- Cloning and sequencing of the amplified 16S rRNA gene amplicon.
- Phylogenetic analysis using aligned 16S rRNA gene sequences.
Main Results:
- The 16S rRNA gene sequence of U. gallorale was successfully obtained.
- Phylogenetic analysis revealed that U. gallorale is most closely related to Ureaplasma urealyticum.
- Distinct evolutionary divergence was observed between U. gallorale and other Ureaplasma species.
Conclusions:
- Ureaplasma gallorale shares a closer evolutionary relationship with Ureaplasma urealyticum.
- This finding contributes to understanding the taxonomy and evolution within the Ureaplasma genus.
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