Related Experiment Video
Updated: Aug 9, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
ISGF3 gamma p48, a specificity switch for interferon activated transcription factors
A R Bluyssen1, J E Durbin, D E Levy
1Department of Pathology, NYU School of Medicine, NY 10016, USA.
Abstract:
Interferon (IFN) induces gene expression by phosphorylating latent transcription factors of the STAT family. Two different STAT multimeric complexes that bind distinct enhancer elements are activated by IFN alpha and IFN gamma, dictated by the DNA-binding protein ISGF3 gamma p48. This protein, a member of the IFN regulatory factor (IFR) family, acts as an adaptor protein to redirect STAT multimers from their intrinsic palindromic sequence specificity to interactions with a composite element composed of an IRF site juxtaposed with a STAT half-site. Sequence similarity within the IRF family suggests that other members could serve as adaptor proteins for transcriptional activators. Recent evidence suggests that PIP (LSIRF) sequesters the Ets protein PU.1 at a composite DNA element lends support to this adaptor hypothesis.
Related Concept Videos
Transcription Factors
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
General Transcription Factors
TGF - β Signaling Pathway
Activation and Inactivation of G Proteins
Inhibitors of Viral Protein Synthesis

