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Related Experiment Videos

Insulin receptor expression in bone

D M Thomas1, D K Hards, S D Rogers

  • 1University of Melbourne, Department of Medicine, St. Vincent's Hospital, Fitzroy Victoria, Australia.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|September 1, 1996
PubMed
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Insulin receptor expression in bone cells increases with osteoblast differentiation. This suggests insulin

Area of Science:

  • Bone Biology
  • Endocrinology
  • Cellular Metabolism

Background:

  • Insulin is recognized as an osteotropic hormone.
  • Regulation of insulin receptor (IR) expression by bone cell differentiation remains uninvestigated.

Purpose of the Study:

  • To investigate insulin receptor distribution and expression in neonatal rat calvaria.
  • To determine if insulin receptor expression correlates with osteoblast differentiation.

Main Methods:

  • Immunohistochemical analysis of neonatal rat calvaria using a monoclonal antibody (CT-1) against the insulin receptor beta-subunit.
  • Enzymatic isolation of calvarial cell subpopulations.
  • Measurement of alkaline phosphatase activity, 125I-insulin binding, and insulin-stimulated 2-deoxyglucose (2-DOG) uptake in cell lines (UMR 201-10B and UMR 106-01).

Related Experiment Videos

  • Northern blot analysis of IR mRNA and GLUT1 mRNA expression.

    • Main Results:

    • Heterogeneous distribution of insulin receptors in neonatal rat calvaria, with higher binding in mature osteoblasts compared to periosteal tissues and osteocytes.
    • Correlation between alkaline phosphatase activity, insulin-stimulated 2-DOG uptake, and 125I-insulin binding in calvarial cell subpopulations.
    • Mature osteoblast cell line (UMR 106-01) exhibited higher IR mRNA levels, insulin binding, and insulin-stimulated glucose uptake compared to preosteoblast-like cells (UMR 201-10B).
    • Insulin treatment upregulated GLUT1 mRNA expression by 3-fold in UMR 106-01 cells.

    Conclusions:

    • Insulin receptor expression is heterogeneous in neonatal rat calvaria and correlates with the stage of osteoblast differentiation.
    • These findings suggest that insulin receptor expression is regulated by osteoblast differentiation, similar to its regulation in muscle and fat tissues.