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Characterization of interactions between bile salts and drugs by micellar electrokinetic capillary chromatography.
M A Schwarz1, R H Neubert, G Dongowski
1Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Halle/Saale, Germany.
Purpose:
The general properties of micellar electrokinetic capillary chromatography (MECC) were utilized to characterize the strength of interactions between bile salts and biological active substances.
Methods:
For that purpose various bile salts were used as micellar pseudostationary phase in the background electrolyte. Furthermore, a physicochemical model was applied and the effective partition coefficients between micellar and water phase were calculated in order to evaluate the strength of interactions between bile acids and the drugs.
Results:
It was found that the interactions between the selected drugs and bile salts depend both on the lipohilicity of the drugs and on the charge of the components. Only hydrophobic, cationic drugs such as quinine and propranolol are able to interact with these surface active agents.
Conclusions:
MECC is a valuable method to characterize interactions such occurring between drugs and bile salts.