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A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution
C E Burnham1, B Buerk, C Schmidt
1Division of Nephrology and Hypertension, University of Cincinnati College of Medicine, OH 45267-0585, USA. charles.burnham@ucbeh.san.uc.edu
Biochimica Et Biophysica Acta
|October 2, 1996
Abstract:
We report the cloning of a 2.2 kb cDNA encoding a Na(+)-and Cl- dependent betaine/GABA (gamma-aminobutyric acid) transporter from rat liver poly(A+) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe. Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.