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Immunochemical studies on human, bovine and pig brain regulatory subunits of cAMP-dependent protein kinase type II
E V Sveshnikova1, N A Alexandrova, I D Grozdova
1Research Center of Molecular Diagnostics & Therapy, Moscow, Russia.
Biochemistry and Molecular Biology International
|August 1, 1996
Abstract:
Regulatory subunits type II (RII) purified from human, pig and bovine brains were compared using II monoclonal antibodies (MoAb) against bovine brain RII. Bovine RII has at least 5 antigenic sites located in the N-terminal 1-110 residues. Immunochemical difference detected between human and animal RII was more pronounced than between pig and bovine RII. Certain MoAb influenced R-cAMP binding and holoenzyme formation. RII of the three species responded to MoAb attachment in a similar fashion. The results suggest that anchoring of neural protein kinase via the N-terminal part of RII may influence the enzyme activity.