A novel physiological function for platelet-derived growth factor-BB in rat dermis
1Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
The Journal of Physiology
|August 15, 1996
Summary
Platelet-derived growth factor-BB (PDGF-BB) and alpha 2 beta 1-integrin regulate interstitial fluid pressure (Pif). Blocking alpha 2 beta 1-integrin lowers Pif, but PDGF-BB administration normalizes it, revealing a novel role for PDGF-BB in Pif control.
Area of Science:
- Physiology
- Cell Biology
- Biochemistry
Background:
- Interstitial fluid pressure (Pif) is a critical physiological parameter.
- The regulation of Pif by cellular adhesion receptors and growth factors is not fully understood.
Purpose of the Study:
- To investigate the role of platelet-derived growth factor-BB (PDGF-BB) and alpha 2 beta 1-integrin in controlling rat skin interstitial fluid pressure (Pif).
Main Methods:
- Interstitial fluid pressure (Pif) was measured in rat skin using glass capillaries and a servocontrolled counter-pressure system.
- Subdermal injections of anti-alpha 2 beta 1-integrin antibodies and PDGF-BB were administered.
- Dextran anaphylaxis was used as a model to induce changes in Pif.
Main Results:
- Blocking alpha 2 beta 1-integrin with antibodies significantly decreased Pif, making it more negative.
- Co-administration of PDGF-BB with anti-alpha 2 beta 1-integrin antibodies abolished the effect of the antibodies.
- PDGF-BB administration normalized Pif during dextran-induced anaphylaxis, while PDGF-AA and basic fibroblast growth factor had no effect.
Conclusions:
- Alpha 2 beta 1-integrin plays a role in controlling interstitial fluid pressure (Pif).
- Platelet-derived growth factor-BB (PDGF-BB) can counteract the effects of alpha 2 beta 1-integrin blockade on Pif.
- These findings suggest PDGF-BB actively participates in regulating Pif by modulating connective tissue cell interactions with the extracellular matrix.
