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Depolarization with high-K+ causes Ca(2+)-independent but partially Cl(-)-dependent glutamate release in rat
1Tsukuba Research Laboratories, Upjohn Pharmaceuticals Limited, Ibaraki, Japan.
Neuroscience Research
|August 1, 1996
Abstract:
We studied the neurotoxic glutamate release induced by high-K+ depolarization in rat hippocampal slice cultures. Depolarization with 90 mM K+ for 30 min caused a significant, three-fold increase in glutamate release. This release was not inhibited by removing extracellular Ca2+, but was significantly inhibited by replacement of extracellular Cl- with SO4(2-). These findings suggest that glutamate is released by mechanisms other than conventional vesicular release under the high-K+ condition.