Effect of cisplatin and cis-platinum (II) phosphonate complex on murine mast cells

We investigated the effect of two cis-platinum (II) complexes, cis-[PtCl2(NH3)2] (cisplatin) and cis-[PtCl2(4-pmpe)2] (4-pmpe stands for diethyl 4-pyridylmethylphosphonate), which was recently synthetized in our laboratory, on murine mast cells. We noticed that both tested compounds were able to evoke histamine release from murine mast cells. The histamine secretion was dependent on the concentration of compound and on the time and temperature of the reaction. It was also dependent on metabolic energy (the reaction was diminished in a medium without glucose and abolished in the presence of 2-deoxyglucose). The results indicate that cis-platinum (II) complexes activate mast cells to secrete histamine via a non-cytotoxic, active secretory process.