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Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1
L Knoepfel1, O Georgiev, P Nielsen
1Institut für Molekularbiologie II der Universität Zürich, Switzerland.
Biological Chemistry Hoppe-Seyler
|February 1, 1996
Abstract:
From a murine B-cell cDNA-library we have cloned a cDNA encoding the murine B-cell specific coactivator mBob1. The protein is the murine homologue to the recently described human coactivator Bob1 (hBob1), also referred to as OBF-1 or OCA-B. We have also characterized the genomic mBob1 clone. Analysis of its intron-exon structure has allowed identification of a C-terminal splice variant. mBob1 is B-cell restricted, and is found in all B-cell lines representing different stages of B-cell differentiation. mBob1 interacts with the octamer transcription factors Oct-1 and Oct-2 and stimulates transcription mediated by these factors.