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Spontaneous CD30 mRNA expression in peripheral blood mononuclear cells from atopic patients with high IgE serum

S Esnault1, N Benbernou, F Lavaud

  • 1Laboratoire d'Immunologie, UFR de Pharmacie, Université de Reins, France.

Clinical and Experimental Immunology
|October 1, 1996
PubMed

Insights

CD30 molecule expression is significantly higher in atopic patients compared to controls. This CD30 mRNA expression in peripheral blood mononuclear cells correlates with IgE levels, suggesting a role in allergic diseases.

Area of Science:

  • Immunology
  • Allergy Research

Background:

  • CD30 is a molecule found on normal B and T lymphocytes.
  • Allergic diseases involve complex immune system dysregulation.

Purpose of the Study:

  • To investigate CD30 expression and release in atopic individuals.
  • To compare CD30 with IL-4 and CD23-alpha/beta in peripheral blood mononuclear cells (PBMC).

Main Methods:

  • Analysis of CD30 mRNA expression in PBMC from atopic subjects and controls.
  • ELISA to detect soluble CD30 (sCD30).
  • Study of purified B cells from allergic patients.

Main Results:

  • CD30 mRNA was absent in control PBMC but significantly present in 6/11 atopic patients.
  • No significant soluble CD30 (sCD30) was detected in either group.
  • CD30 mRNA expression in allergic patients' PBMC positively correlated with serum IgE levels (r = 0.79, P = 0.003).
  • CD30 was primarily expressed on CD19+ B cells in allergic patients.

Conclusions:

  • CD30 molecule expression is notably elevated in atopic individuals.
  • A strong correlation exists between CD30 mRNA levels and IgE serum concentrations.
  • The CD30 molecule likely plays a functional role in the IgE response within allergic diseases.

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