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Toward quality assurance for metaphase FISH: a multi-center experience
G Dewald1, R Stallard, P I Bader
1Cytogenetics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
American Journal of Medical Genetics
|September 6, 1996
Summary
Proficiency testing for fluorescent in situ hybridization (FISH) probes is feasible across multiple centers. Collaboration can effectively evaluate probe efficacy and costs, despite initial inexperience challenges.
Area of Science:
- Cytogenetics
- Molecular Biology
- Clinical Diagnostics
Background:
- Fluorescent in situ hybridization (FISH) is increasingly used in clinical cytogenetics.
- No existing organizations sponsor multi-center evaluations of FISH probe efficacy.
Purpose of the Study:
- To assess the feasibility of multi-center proficiency testing for FISH probes.
- To evaluate the efficacy and cost-effectiveness of FISH probes across different laboratories.
Main Methods:
- 23 laboratories participated in a multi-center study using SNRPN and control locus FISH probes.
- A blind study involved scoring metaphases from normal and abnormal specimens.
- Hybridization efficiency and diagnostic accuracy were assessed.
Main Results:
- High hybridization efficiency was observed (median 97.0-97.8%).
- Correct SNRPN patterns were identified in 92% of challenges (5/5 or 4/5 metaphases).
- Seven diagnostic errors (1%) occurred, primarily due to inexperience; batch processing reduced analysis time.
Conclusions:
- Multi-center proficiency testing for FISH is achievable.
- Collaboration enables effective probe evaluation and cost analysis.
- Addressing inexperience is key to improving FISH accuracy in clinical settings.