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Related Experiment Videos

Tight junction dynamics in the frog urinary bladder

F Lacaz-Vieira1, B Kachar

  • 1Department of Physiology and Biophysics, University of Säo Paulo, Rockville, MD 20850, USA.

Cell Adhesion and Communication
|July 1, 1996
PubMed
Summary
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Apical calcium (Ca2+) reseals disrupted tight junctions (TJs) in frog urinary bladders, independent of cell entry. Protein kinase C (PKC) also plays a key role in TJ repair.

Area of Science:

  • Epithelial physiology
  • Cell biology
  • Membrane transport

Background:

  • Tight junctions (TJs) regulate paracellular permeability in epithelial tissues.
  • Previous studies demonstrated Ca2+ can reseal TJs in frog skin.
  • The role of apical Ca2+ in TJ resealing in frog urinary bladders was unexplored.

Purpose of the Study:

  • To investigate the role of apical Ca2+ in resealing experimentally disrupted TJs in frog urinary bladders.
  • To determine the mechanism by which apical Ca2+ promotes TJ resealing.
  • To explore the involvement of Protein Kinase C (PKC) in TJ assembly and recovery.

Main Methods:

  • Experimental disruption of TJs using high transepithelial potentials, hypertonic solutions, or BaSO4 deposition.
  • Assessment of TJ resealing by monitoring changes in conductance.

Related Experiment Videos

  • Pharmacological manipulation using Ca2+ channel blockers, PKC inhibitor (H7), and PKC activator (diC8).

    • Main Results:

    • Apical Ca2+ addition promoted TJ resealing in frog urinary bladders, irrespective of Ca2+ channel blockers.
    • TJ resealing appears to be mediated by direct interaction of apical Ca2+ with zonula adhaerens receptors (E-cadherins).
    • PKC activation/inhibition significantly affected TJ recovery, with H7 promoting conductance recovery and diC8 inhibiting it.

    Conclusions:

    • Apical Ca2+ is crucial for TJ resealing in frog urinary bladders, acting directly on the junctions.
    • TJ resealing does not rely on Ca2+ influx through apical membrane channels.
    • PKC signaling is essential for the regulation and assembly of TJs in this epithelium.