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Structure, tissue distribution and genomic organization of the murine RRM-type RNA binding proteins TIA-1 and TIAR
A R Beck1, Q G Medley, S O'Brien
1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Abstract:
TIA-1 and TIAR are RNA binding proteins of the RNA recognition motif (RRM)/ribonucleoprotein (RNP) family that have been implicated as effectors of apoptotic cell death. We report the structures of murine TIA-1 and TIAR (mTIA-1 and mTIAR) deduced from cDNA cloning, the mRNA and protein tissue distribution of mTIA-1 and mTIAR, and the exon-intron structures of the mTIA-1 and mTIAR genes. Both mTIA-1 and mTIAR are comprised of three approximately 100 amino acid N-terminal RRM domains and a approximately 90 amino acid C-terminal auxiliary domain. This subfamily of RRM proteins is evolutionarily well conserved; mTIA-1 and mTIAR are 80% similar to each other, and 96 and 99% similar to hTIA-1 and hTIAR, respectively. The overall exon-intron structures of the mTIA-1 and mTIAR genes are also similar to each other, as well as to the human TIA-1 gene structure. While Northern blot analysis reveals that mTIA-1 and mTIAR mRNAs have a broad tissue distribution, mTIA-1 and mTIAR proteins are predominantly expressed in brain, testis and spleen. At least two isoforms of both mTIA-1 and mTIAR are generated by alternative splicing. Murine TIA-1 isoforms including or lacking the exon 5 encoded sequences are expressed at a ratio of approximately 1:1, whereas mTIAR isoforms including or lacking the 5'-end of exon 3 sequences are expressed in a approximately 1:6 ratio. Molecular characterization of murine TIA-1 and TIAR RNA binding proteins provides the basis for a genetic analysis of the functional roles of these proteins during mammalian development.
Insights
Murine TIA-1 and TIAR RNA binding proteins, crucial for apoptosis, share conserved structures and broad mRNA distribution. Protein expression is concentrated in brain, testis, and spleen, with alternative splicing generating distinct isoforms.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- TIA-1 and TIAR are RNA binding proteins involved in apoptotic cell death.
- These proteins belong to the RNA recognition motif (RRM)/ribonucleoprotein (RNP) family.
Purpose of the Study:
- To characterize the structures of murine TIA-1 and TIAR (mTIA-1 and mTIAR).
- To determine the mRNA and protein tissue distribution of mTIA-1 and mTIAR.
- To elucidate the exon-intron structures of the mTIA-1 and mTIAR genes.
Main Methods:
- cDNA cloning was used to deduce the structures of mTIA-1 and mTIAR.
- Northern blot analysis was performed to assess mRNA tissue distribution.
- Protein expression patterns were analyzed across various tissues.
Main Results:
- Both mTIA-1 and mTIAR possess three N-terminal RRM domains and a C-terminal auxiliary domain.
- These proteins are highly conserved across species and highly similar to each other.
- While mRNAs are broadly distributed, proteins are predominantly found in brain, testis, and spleen.
- Alternative splicing generates at least two isoforms for both mTIA-1 and mTIAR.
Conclusions:
- Molecular characterization of murine TIA-1 and TIAR provides a foundation for genetic studies.
- Understanding these RNA binding proteins is crucial for analyzing their roles in mammalian development and apoptosis.