Level of myelopoiesis in the bone marrow is influenced by intestinal flora

Mice were orally given kanamycin (50 mg/day/ mouse) for 1 or 2 weeks. Almost all bacteria in the intestine were eliminated within a week. In parallel with this elimination, the level of granulocytes in the bone marrow decreased from 41 to 31%. Since the total number of bone marrow cells decreased as well (up to one-third), the decrease in the level of granulocyte generation in the bone marrow was substantial. Kanamycin is not absorbed from the intestine into the body; namely, it does not have a direct effect on the bone marrow. It is speculated that the interaction of granulocytes or epithelial cells with intestinal bacteria accelerates the production of free radicals and superoxids, some cytokines, or unknown factors and that such substances then stimulate the generation of granulocytes in the bone marrow. The present results suggest that intestinal flora and other resident bacteria are important for maintaining the level of myelopoiesis in the bone marrow.