Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse signaling via CD30 ligand

S R Wiley1, R G Goodwin, C A Smith

  • 1Immunex Corporation, Seattle, WA 98101, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|October 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymorphisms in drug-metabolizing enzymes as modifiers of cancer risk.

Clinical chemistry·1995
Same author

CD30-mediated signaling promotes the development of human T helper type 2-like T cells.

The Journal of experimental medicine·1995
Same author

Objective measurement of the benefit of walking sticks in peripheral vestibular balance disorders, using the Sway Weigh balance platform.

The Journal of laryngology and otology·1995
Same author

Arthritis and perceptions of quality of life: an examination of positive and negative affect in rheumatoid arthritis patients.

Health psychology : official journal of the Division of Health Psychology, American Psychological Association·1995
Same author

Diastolic time: an important determinant of regional arterial blood flow.

The American journal of physiology·1995
Same author

Are we vaccinating too much?

Journal of the American Veterinary Medical Association·1995

Tumor necrosis factor (TNF) superfamily member CD30 ligand (CD30L) can directly signal to the cell expressing it when cross-linked. This "reverse signaling" suggests TNF family members generally signal bidirectionally.

Area of Science:

  • Immunology
  • Cell Signaling
  • Molecular Biology

Background:

  • CD30 ligand (CD30L) is a type II membrane protein in the TNF superfamily.
  • Conserved cytoplasmic domains suggest a potential signaling role for CD30L.
  • TNF family members often exhibit bidirectional signaling.

Purpose of the Study:

  • To investigate if cross-linked CD30L can directly signal to the ligand-bearing cell.
  • To explore the phenomenon of "reverse signaling" in CD30L.
  • To determine if TNF family members universally signal bidirectionally.

Main Methods:

  • Cross-linking CD30L on neutrophils using a mAb or CD30-Fc fusion protein.
  • Measuring IL-8 production and oxidative burst in neutrophils.
  • Inducing CD30L expression on T cells via anti-CD3 antibody.

Related Experiment Videos

  • Assessing T cell metabolic activity, proliferation, and IL-6 production upon CD30L cross-linking.

    • Main Results:

    • Cross-linked CD30L induced IL-8 production and oxidative burst in neutrophils.
    • Neutrophils express CD30L but not CD30, ruling out indirect effects.
    • Cross-linked CD30L triggered increased metabolic activity, proliferation, and IL-6 production in T cells.
    • CD30L demonstrated direct signaling capacity to the ligand-bearing cell.

    Conclusions:

    • Cross-linked CD30L can directly transduce signals to the cell expressing it.
    • This reverse signaling supports the hypothesis of bidirectional communication for TNF family members.
    • The distinction between ligand and receptor may be blurred within the TNF superfamily.