Xenogeneic moose (Alces alces) bone morphogenetic protein (mBMP)-induced repair of critical-size skull defects in sheep

A standardized skull defect in adult sheep was used to test the healing capacity of xenogeneic, partially purified, moose-derived bone morphogenetic protein (mBMP) extracted from the fresh long bones of moose (Alces alces) calves. An amount of 52 mg of mBMP mixed with 13 mg of purified type IV collagen (5:1) (mBMP/COL) in gelatin capsules was implanted into six 22-mm-diameter skull defects in adult sheep for comparison with six defects implanted with fresh autogenous bone marrow (BM) and six other controls implanted with a gelatin capsule containing 13 mg of type IV collagen (C). The amount of new bone formed was quantified from radiographs by computerized image analysis and histology. The healing percentage in the mBMP/COL group was significantly higher (93.18 +/- 4.51%) than in the BM (33.17 +/- 20.05%) or C group (31.32 +/- 17.41%) at 16 weeks after implantation. The difference between BM and C was not statistically significant. The level of anti-BMP antibody in the serum showed a significant increase in the group implanted with mBMP, but returned to normal after 6 weeks. The experiment demonstrated that xenogeneic mBMP possesses a strong osteoinductive capacity and weak immunogenicity.