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Related Experiment Videos

Calcium buffering is required to maintain bone stiffness in saline solution

M B Gustafson1, R B Martin, V Gibson

  • 1Orthopaedic Research Laboratories, School of Medicine, University of California at Davis, 95616, USA.

Journal of Biomechanics
|September 1, 1996
PubMed
Summary

Prolonged saline storage can weaken bone elastic modulus. Buffering saline with calcium ions prevents this mineral dissolution, preserving bone mechanical properties during testing or storage.

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Area of Science:

  • Biomaterials Science
  • Orthopedic Research
  • Materials Science

Background:

  • Bone mineral dissolution can affect mechanical properties.
  • Storage and testing conditions may alter bone integrity.
  • Understanding saline effects is crucial for bone research.

Purpose of the Study:

  • To determine if mineral dissolution in saline affects bone elastic modulus.
  • To investigate the impact of calcium ion concentration on bone stability.
  • To establish optimal saline conditions for bone specimen preservation.

Main Methods:

  • Equine bone specimens were soaked in normal saline with varying CaCl2 concentrations.
  • Equilibrium calcium ion concentration was determined.
  • Elastic moduli of bone beams were measured non-destructively before and after soaking in normal or calcium-buffered saline.

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Main Results:

  • The equilibrium calcium ion concentration for bone was 57.5 mg/L.
  • Bone specimens in normal saline showed a significant 2.4% decrease in elastic modulus.
  • Bone specimens in calcium-buffered saline exhibited no significant change in elastic modulus.

Conclusions:

  • Prolonged exposure to normal saline causes significant bone mineral dissolution and reduces elastic modulus.
  • Calcium-buffered saline prevents mineral dissolution and preserves bone elastic modulus.
  • Calcium-buffered saline is recommended for testing or storing bone specimens to maintain mechanical integrity.