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Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

C S Unterkircher1, S C Yazaki, M T Shimizu

  • 1School of Dentistry, Department of Pathology, São José dos Campos, UNESP, São Paulo, Brazil.

Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology
|July 1, 1996
PubMed
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Researchers investigated circulating immune complexes (CIC) in paracoccidioidomycosis (PCM) patients. They identified specific fungal antigens bound within these complexes, aiding in understanding disease-related immune responses.

Area of Science:

  • Mycology
  • Immunology
  • Infectious Diseases

Background:

  • Paracoccidioidomycosis (PCM) is a systemic fungal infection caused by Paracoccidioides brasiliensis.
  • Circulating immune complexes (CIC) are implicated in the immunopathology of various infectious diseases.
  • Understanding the composition of CIC in PCM is crucial for diagnosing and managing the disease.

Purpose of the Study:

  • To analyze the components of circulating immune complexes (CIC) in patients with paracoccidioidomycosis (PCM).
  • To identify specific fungal antigens bound within these CIC.
  • To elucidate the role of high-affinity antibody-antigen interactions in PCM pathogenesis.

Main Methods:

  • Circulating immune complexes (CIC) were isolated from serum samples of PCM patients and healthy controls.

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  • CIC were dissociated, and free antibodies were tested for reactivity against P. brasiliensis antigens.
  • Dissociated CIC components were fractionated using Sephacryl S-300 chromatography.
  • Fractions containing potential antigens were purified using an affinity column with anti-gp43 monoclonal antibody.
  • ECL-Western blotting was employed to detect and characterize polypeptide components.

    • Main Results:

    • Limited reactivity of free antibodies from PCM-CIC suggested high-affinity binding to fungal components.
    • Fractionation and affinity purification identified two specific polypeptides within the CIC.
    • These polypeptides had apparent molecular masses of 43 kDa and 62 kDa.
    • The 43 kDa polypeptide is likely related to the known gp43 antigen of P. brasiliensis.

    Conclusions:

    • Circulating immune complexes in PCM patients contain fungal antigens, notably polypeptides of 43 kDa and 62 kDa.
    • The high-affinity binding of antibodies within CIC suggests a significant role in the host's immune response to P. brasiliensis.
    • These findings contribute to understanding the immune mechanisms underlying paracoccidioidomycosis.