Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A spontaneously immortalized mouse microglial cell line expressing CD4

F Alliot1, M C Marty, D Cambier

  • 1CNRS UPR 9035 Développement & Immunité du Système Nerveux Central, Paris, France.

Brain Research. Developmental Brain Research
|August 20, 1996
PubMed
Summary

Researchers developed a novel microglial cell line, C8-B4, from mouse cerebellum cultures. This unique cell line synthesizes CD4 and releases glutamate, offering new research avenues in neurobiology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lower insulin sensitivity is related to lower relative muscle cross-sectional area, lower muscle density and lower handgrip force in young and middle aged non-diabetic men.

Journal of musculoskeletal & neuronal interactions·2016
Same author

Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons.

The Science of the total environment·2016
Same author

Determination of phthalates and their by-products in tissues of roach (Rutilus rutilus) from the Orge river (France).

Environmental science and pollution research international·2014
Same author

Association between insulin resistance, lean mass and muscle torque/force in proximal versus distal body parts in healthy young men.

Journal of musculoskeletal & neuronal interactions·2014
Same author

Persistent organic pollutants in benthic and pelagic organisms off Adélie Land, Antarctica.

Marine pollution bulletin·2013
Same author

Changes in microcirculation of the trapezius muscle during a prolonged computer task.

European journal of applied physiology·2012

Area of Science:

  • Neuroscience
  • Cell Biology
  • Immunology

Background:

  • Microglia are key immune cells in the central nervous system.
  • Establishing well-characterized microglial cell lines is crucial for studying their function.
  • Previous work reported astroglial cell lines derived from similar cultures.

Purpose of the Study:

  • To characterize a newly derived microglial cell clone, C8-B4.
  • To investigate novel molecular markers and functions of this microglial clone.
  • To assess its potential as a model for microglial research.

Main Methods:

  • Derivation of microglial clone C8-B4 from 8-day mouse cerebellum organ culture.
  • Immunophenotyping using classical microglial markers (MAC1, F4/80, 2-4G2).

Related Experiment Videos

  • Analysis of monocytic lineage differentiation antigens and CD4 expression.
  • Assessment of glutamate production and release.

    • Main Results:

    • The C8-B4 clone expresses established microglial markers.
    • It lacks early monocytic lineage differentiation antigens, indicating a committed microglial precursor origin.
    • C8-B4 synthesizes the CD4 molecule, a novel finding for microglial cell lines.
    • This clone produces and releases significant amounts of glutamate.

    Conclusions:

    • The C8-B4 clone represents a well-defined microglial cell line with unique characteristics.
    • Its ability to synthesize CD4 and release glutamate opens new possibilities for studying microglial-neuronal interactions.
    • This cell line serves as a valuable tool for neuroinflammatory and neurodegenerative disease research.