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Related Experiment Videos

Tear tryptase levels and allergic conjunctivitis

L Magrini1, S Bonini, M Centofanti

  • 1Institute of I Clinic e Medica, University of Rome La Sapienza, Italy.

Allergy
|August 1, 1996
PubMed
Summary
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Vernal keratoconjunctivitis (VKC) patients show elevated tear tryptase levels, indicating mast cell activation even during remission. Allergic conjunctivitis patients exhibit increased tryptase post-allergen challenge, primarily in the early phase.

Area of Science:

  • Ophthalmology
  • Immunology
  • Allergy

Background:

  • Mast cells are key players in allergic inflammation.
  • Tryptase is a neutral protease released by activated mast cells.
  • Vernal keratoconjunctivitis (VKC) is a severe ocular allergic disease.

Purpose of the Study:

  • To measure tear tryptase levels in VKC patients during remission.
  • To compare tear tryptase levels between VKC, allergic conjunctivitis, and healthy controls.
  • To investigate mast cell activation in allergic conjunctivitis following allergen challenge.

Main Methods:

  • Solid-phase radioimmunoassay was used to measure tryptase in tears.
  • Tear samples were collected from VKC patients, allergic conjunctivitis patients, and healthy controls.

Related Experiment Videos

  • Allergic conjunctivitis patients underwent topical allergen challenge.

    • Main Results:

    • Tear tryptase levels were significantly higher in VKC patients compared to allergic conjunctivitis patients and controls.
    • Tryptase levels in allergic conjunctivitis patients increased 20 minutes after allergen challenge but not after 6 hours.
    • Elevated tear tryptase in VKC suggests mast cell activation even in remission.

    Conclusions:

    • VKC is associated with elevated tear tryptase, indicating ongoing mast cell activation.
    • Mast cell activation in allergic conjunctivitis is evident in the early phase of the allergic reaction.
    • Tear tryptase measurement is a valuable biomarker for ocular allergic inflammation.