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Spatiotemporal expression of CD15 in the developing chick retina
C Andressen1, K Moertter, J K Mai
1Institute of Neuroanatomy, Heinrich Heine University, Düsseldorf, Germany.
Insights
CD15 expression in the developing chick retina, particularly in amacrine, bipolar, and ganglion cells, follows a specific spatiotemporal pattern. This pattern correlates with the establishment of synaptic connections, revealing key developmental insights.
Area of Science:
- Neuroscience
- Developmental Biology
- Glycobiology
Background:
- The carbohydrate epitope CD15 plays a role in cellular differentiation and recognition.
- Understanding the spatiotemporal expression of CD15 is crucial for elucidating retinal development.
Purpose of the Study:
- To investigate the spatiotemporal distribution of CD15 in the developing chick retina.
- To correlate CD15 expression patterns with the differentiation of retinal cell types and synaptic development.
Main Methods:
- Immunohistochemistry was used to visualize CD15 expression in retinal sections.
- Western blotting was employed to identify CD15-positive glycoproteins.
Main Results:
- CD15 expression initiated at embryonic day 13 (E13) in the inner nuclear layer (INL) and ganglion cell layer (GCL).
- Stratified amacrine cells showed increasing CD15 expression from E13-E16, with diffuse subtypes appearing at E15.
- Three types of CD15-positive ganglion cells were identified by E15, and an adult-like pattern was established by E19.
- CD15-positive glycoproteins (20, 32, 34 kDa) were detected from E16 to postnatal day 5 (P5).
Conclusions:
- The spatiotemporal expression of CD15 in the chick retina is linked to the maturation of amacrine, bipolar, and ganglion cells.
- CD15 expression patterns correlate with the formation of synaptic contacts during retinal development.
- CD15 serves as a valuable marker for studying cellular differentiation and circuit formation in the developing visual system.
Abstract:
The spatiotemporal distribution pattern of the carbohydrate epitope CD15 was examined in the developing chick retina. CD15 expression appeared for the first time at E13 in the INL and GCL. The developmental profile of the INL, from E13 to E16, showed increasing numbers of stratified amacrine cells, whereas diffuse amacrine subtypes appeared later, beginning at E15. Smaller populations of bipolar cells were seen at E17. Three types of CD15-positive ganglion cells could be differentiated by E15. A gradient in the appearance of identified immunoreactive amacrine cells extended from the dorsotemporal to the ventral and nasal retina. The adult-like pattern of CD15 expression did not become established until E19. Adult-like densities of immunoreactive cells were reached toward the end of the embryonic period between E18 in the dorsotemporal and ventral retina, and E19 in the dorsonasal retina. In the adult-like retina, labelled cells became particularly numerous at its greatest circumference, and were most densely packed in the dorsotemporal retinal quadrant. From E16 to P5, three membrane-bound, CD15-positive glycoproteins of 20, 32 and 34 kDa were identified by Western blots. The time course in the appearance of the membrane-associated CD15 recognition molecule on differentiating amacrine, bipolar and ganglion cells is correlated to the establishment of synaptic contacts.