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Aging and polyamine acetylation in rat kidney

M E Ferioli1, A Sessa, P Tunici

  • 1Centro di Studio sulla Patologia Cellulare, C.N.R., Università di Milano, Milan, Italy.

Biochimica Et Biophysica Acta
|October 7, 1996
PubMed
Summary

Aging in rat kidneys activates polyamine acetylation and interconversion. This process shifts polyamine metabolism towards putrescine formation, influencing kidney function over time.

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Area of Science:

  • Biochemistry
  • Gerontology
  • Nephrology

Background:

  • Polyamines are essential for cell growth and function.
  • Age-related changes in polyamine metabolism can impact kidney health.
  • Understanding these changes is crucial for addressing age-associated kidney dysfunction.

Purpose of the Study:

  • To investigate the age-dependent changes in polyamine acetylation and interconversion in rat kidneys.
  • To elucidate the role of specific enzymes in these metabolic pathways during aging.
  • To determine the implications of these alterations for polyamine levels and kidney function.

Main Methods:

  • Assessed the activity of key enzymes: spermidine/spermine N1-acetyltransferase, spermidine N8-acetyltransferase, and polyamine oxidase.
  • Measured enzyme activities in rat kidney cytosol across different age groups (3, 24, and 36 months).

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  • Monitored polyamine and acetylated polyamine levels in relation to enzyme activity changes.

    • Main Results:

    • Cytosolic spermidine/spermine N1-acetyltransferase activity increased with age, indicating enhanced acetylation.
    • Spermidine N8-acetyltransferase activity also rose, suggesting increased polyamine excretion.
    • Polyamine oxidase activity initially decreased, leading to N1-acetylspermidine accumulation, then increased, correlating with spermidine and putrescine increases and spermine decline.

    Conclusions:

    • Aging activates polyamine acetylation and interconversion pathways in the rat kidney.
    • These changes favor the formation of putrescine, an alternative pathway for spermidine and putrescine.
    • Age-related shifts in polyamine metabolism may contribute to functional changes in the aging kidney.