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Related Experiment Videos

Pharmacologic modulation of delayed-type hypersensitivity in mice

N Yamada1, K Sugasawa

  • 1Pharmaceuticals Laboratory, Yokohama Research Center, Mitsubishi Chemical Corporation, Japan.

Arzneimittel-Forschung
|September 1, 1996
PubMed
Summary

Mast cells and histamine play a key role in delayed-type hypersensitivity (DTH) reactions in mice. Inhibiting mast cell degranulation and histamine 16 hours post-challenge significantly reduced DTH responses.

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Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Delayed-type hypersensitivity (DTH) is a critical immune response.
  • Mast cells are implicated in DTH, but their precise role requires elucidation.

Purpose of the Study:

  • To investigate the role of mast cells in murine DTH reactions.
  • To evaluate the effects of specific pharmacological inhibitors on DTH.

Main Methods:

  • Murine DTH models were established using sheep red blood cells (SRBC).
  • Various drugs, including chlorpheniramine, repirinast, BW-755C, ibuprofen, diclofenac, and phenylbutazone, were administered at different time points post-antigen challenge.
  • The delayed footpad reaction (FPR) was measured to assess DTH severity.

Main Results:

Related Experiment Videos

  • Chlorpheniramine significantly inhibited SRBC-induced DTH when administered 16 hours post-challenge.
  • Repirinast, an inhibitor of mast cell degranulation, dose-dependently inhibited DTH when given 16 hours post-challenge.
  • BW-755C, a lipoxygenase and cyclooxygenase inhibitor, also significantly inhibited DTH at 16 hours post-challenge.
  • Cyclooxygenase inhibitors (ibuprofen, diclofenac, phenylbutazone) showed no significant effect on DTH.

    • Conclusions:

    • Mast cells are involved in murine DTH reactions.
    • Histamine and leukotrienes appear to play significant roles in DTH pathogenesis.
    • The timing of pharmacological intervention is crucial for modulating DTH responses.