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ATP-induced calcium mobilization in glomerular mesangial cells is mediated by P2U purinoceptor
M Takeda1, T Kawamura, M Kobayashi
1Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Abstract:
To identify the functional P2 purinoceptor subtype in glomerular mesangial cells (GMC), polymerase chain reaction coupled with reverse transcription (RT-PCR) were performed, and cytosolic free calcium concentration ([Ca2+]i) was determined. RT-PCR analysis revealed that the molecular identity of P2 purinoceptor localized to GMC was both P2U and P2Y. The rank order of potency in stimulating [Ca2+]i was ATP [symbol: see text] UTP > 2-methylthio-ATP > ADP [symbol: see text] adenosine [symbol: see text] AMP. In addition, cross-desensitization between ATP and UTP occurred. In conclusion, ATP induces increase in [Ca2+]i via P2U purinoceptor in GMC.