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Specific guanylation of Lupinus luteus 5S rRNA at its 3' end in HeLa cell extract
E Wyszko1, Z Szweykowska-Kulinska, M Z Barciszewska
1Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznañ, Poland.
Biochemistry and Molecular Biology International
|August 1, 1996
Abstract:
We have found that Lupinus luteus 5S ribosomal RNA is post-transcriptionally modified at its 3' end by addition of pG catalysed by HeLa cells extract. This extra nucleotide is not encoded within Lupinus luteus 5S rRNA gene. A similar reaction has been previously observed for eukaryotic histidine tRNAs. However an additional guanosine residue was ligated post-transcriptionally at the 5' end of that tRNA.