Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclophosphamide-induced immunological tolerance: an overview

H Mayumi1, M Umesue, K Nomoto

  • 1Department of Cardiovascular Surgery, National Kyushu Medical Center Hospital, Japan.

Immunobiology
|July 1, 1996
PubMed
Summary

This study introduces a cyclophosphamide (CP)-induced tolerance system for long-lasting organ transplant acceptance in mice. The method involves donor cell injection followed by CP treatment, clarifying tolerance mechanisms for potential human application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gap junction protein beta 1 (GJB1) mutations and central nervous system symptoms in X-linked Charcot-Marie-Tooth disease.

Acta neurologica Scandinavica·2003
Same author

[Tracheopulmonary-subcutaneous fistula associated with anaerobic subcutaneous abscess on mechanical ventilator support in a patient with Duchenne muscular dystrophy].

Rinsho shinkeigaku = Clinical neurology·2000
Same author

Current techniques of hyperthermic isolated limb perfusion for malignant melanoma.

Surgery today·2000
Same author

Risk factors for posttransfusion graft versus host disease, mediastinitis, and late cardiac tamponade in heart surgery. Survey of 119 Japanese institutions.

The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi·2000
Same author

Rejection of discordant skin xenografts by CD4- CD8- TCR alpha beta+ cells in CD4- and CD8-deficient mice.

Transplantation proceedings·1999
Same author

Fractionated dosing of cyclophosphamide for establishing long-lasting skin allograft survival, stable mixed chimerism, and intrathymic clonal deletion in mice primed with allogeneic spleen cells.

Transplantation·1997

Area of Science:

  • Immunology
  • Transplantation Biology
  • Experimental Medicine

Background:

  • Inducing long-lasting tolerance to transplanted organs is a major challenge in transplantation.
  • Existing methods often result in transient or incomplete tolerance, necessitating further research into robust tolerance induction strategies.

Purpose of the Study:

  • To investigate a cyclophosphamide (CP)-induced tolerance system for achieving long-lasting allo- and xeno-tolerance to solid organs.
  • To elucidate the sequential mechanisms underlying tolerance induction in the cells-followed-by-CP model.

Main Methods:

  • Utilized a mouse model involving donor cell injection followed by cyclophosphamide (CP) treatment.
  • Analyzed tolerance mechanisms by correlating superantigens with T cell receptor (TCR) V beta segments.

Related Experiment Videos

  • Investigated strategies to overcome barriers in allo- and xeno-transplantation, including optimizing drug dosage, timing, and administration routes.

    • Main Results:

    • The cells-followed-by-CP system successfully induced long-lasting tolerance to various solid organs.
    • Identified key tolerance mechanisms: clonal destruction, peripheral and intrathymic clonal deletion, clonal anergy, and suppressor T cell generation.
    • Optimized parameters like drug choice, dose, and timing were crucial for overcoming transplantation barriers and achieving sustained skin tolerance.

    Conclusions:

    • The CP-induced tolerance system offers a promising approach for long-lasting organ acceptance in transplantation.
    • Understanding the intricate mechanisms of tolerance is vital for refining protocols and improving outcomes.
    • This model holds potential for application in human transplantation, addressing critical unmet needs.