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Nucleotide sequence of the matrix protein gene of a subgroup B avian pneumovirus

J S Randhawa1, C R Pringle, A J Easton

  • 1Department of Biological Sciences, University of Warwick, Coventry, UK.

Virus Genes
|January 1, 1996
PubMed

Insights

The matrix protein gene sequence of avian pneumovirus subgroup B was determined. This sequence analysis can differentiate between avian pneumovirus subgroups A and B.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Avian pneumovirus (APV) causes respiratory illness in birds.
  • APV is classified into subgroups A and B.
  • The matrix protein is a key component of APV structure and replication.

Purpose of the Study:

  • To determine the nucleotide sequence of the matrix protein gene of avian pneumovirus subgroup B.
  • To compare the subgroup B matrix protein gene with that of subgroup A.
  • To assess the utility of the matrix protein gene for differentiating APV subgroups.

Main Methods:

  • Nucleotide sequencing of the APV subgroup B matrix protein gene.
  • Homology analysis comparing subgroup B with subgroup A sequences.
  • Bioinformatic analysis of protein domains.

Main Results:

  • The nucleotide sequence of the avian pneumovirus subgroup B matrix protein gene was successfully determined.
  • A 73.5% homology was observed between the matrix protein genes of APV subgroups A and B.
  • Differences were predominantly found in the third codon position.
  • The APV matrix protein conserves a hydrophobic domain common to other pneumoviruses.

Conclusions:

  • The matrix protein gene sequence provides a reliable method for differentiating between avian pneumovirus subgroups A and B.
  • Sequence analysis of the matrix protein gene is valuable for APV subtyping and diagnostics.

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