Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between complement proteins C5b-7 and erythrocyte membrane sialic acid

P Marshall1, A Hasegawa, E A Davidson

  • 1Dermatology Service, Veterans Affairs Medical Center, New York 10016, USA.

The Journal of Experimental Medicine
|October 1, 1996
PubMed
Summary

Sialic acid on red blood cells initially hinders complement C5b6 binding but surprisingly enhances membrane attack once initiated. This reveals a dual role for sialic acid in the complement cascade.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of sialyl Lewis X ganglioside analogues containing modified L-fucose residues.

Carbohydrate research·1995
Same author

[Blood levels of cyclosporine, acute rejections and the prognosis of the allografts in pediatric renal allograft recipients].

Nihon Hinyokika Gakkai zasshi. The japanese journal of urology·1995
Same author

Synthetic and structural studies of alpha-sialyl-(2-->6) and alpha-sialyl-(2-->3) 1-deoxynojirimycin derivatives potentially useful for biomedical applications.

Carbohydrate research·1995
Same author

Differentiation between nodules and end-on vessels using a convolution neural network architecture.

Journal of digital imaging·1995
Same author

Neuronal expression of a minor monosialosyl ganglioside GM1b in rat brain: immunochemical characterization using a specific monoclonal antibody.

Neuroscience research·1995
Same author

Synthesis of a sialyl Lewis X ganglioside analogue containing N-glycolyl in place of the N-acetyl group in the N-acetylneuraminic acid residue.

Bioscience, biotechnology, and biochemistry·1995

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • The complement system's membrane attack complex (MAC) formation is crucial for cell lysis.
  • Initial steps involve C5b6, C7 interaction with cell membranes to form C5b-7.
  • Sialic acid's role in this early membrane attack phase is not fully understood.

Purpose of the Study:

  • To investigate the specific role of sialic acid residues in C5b-7 assembly on erythrocyte membranes.
  • To elucidate the binding interactions of complement proteins C5b6, C5, and C6 with erythrocyte surface molecules.

Main Methods:

  • Studied C5b6 binding to glycophorin on erythrocyte membranes.
  • Investigated the effect of desialylation and sialic acid masking (using mAb F(ab) fragments) on C5b6 binding.

Related Experiment Videos

  • Assessed inhibition of C5b-7 deposition by gangliosides and asialogangliosides.

    • Main Results:

    • C5b6 specifically binds to glycophorin; C5 or C6 do not.
    • Desialylation of glycophorin abolishes C5b6 binding.
    • Masking or removing sialic acid from glycophorin inhibits complement lysis.
    • Gangliosides inhibit C5b-7 deposition, but asialogangliosides do not.

    Conclusions:

    • C5b6 initially binds to erythrocyte membranes via ionic interactions with sialylated molecules.
    • A model is proposed where C7 binding to C5b6 disrupts ionic interactions, exposing hydrophobic domains.
    • Sialic acid plays a paradoxical role: inhibiting initial C5b6 generation but enhancing lysis once the membrane attack pathway is initiated.