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Related Experiment Videos

CD5 is a potential selecting ligand for B cell surface immunoglobulin framework region sequences

R Pospisil1, M G Fitts, R G Mage

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

The Journal of Experimental Medicine
|October 1, 1996
PubMed
Summary

Rabbit B lymphocytes express CD5, a glycoprotein whose function is unknown. We found that immunoglobulin fragments interact with CD5, suggesting a role in B cell selection and potential links to autoimmune diseases.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • In rabbits, nearly all B lymphocytes express the glycoprotein CD5, unlike in mice and humans where only a small proportion do.
  • CD5+ B cells in rabbits appear to develop early and are maintained by self-renewal.
  • The precise function of CD5 on B cells remains largely unknown.

Purpose of the Study:

  • To investigate the function of CD5 on rabbit B lymphocytes.
  • To determine if specific immunoglobulin structures interact with CD5.
  • To explore the potential role of CD5-immunoglobulin interactions in B cell development and disease.

Main Methods:

  • Utilized F(ab")2 fragments, particularly those with VHa2 framework region determinants.
  • Tested for specific interactions between F(ab")2 fragments and B cell-surface CD5.

Related Experiment Videos

  • Investigated inhibition of these interactions using anti-CD5 antibodies.
  • Assessed binding of immobilized F(ab")2 fragments to CD5 in appendix cell lysates.

    • Main Results:

    • F(ab")2 fragments, especially those with VHa2 determinants, specifically interact with the B cell-surface glycoprotein CD5.
    • This interaction is inhibitable by anti-CD5 antibodies.
    • Immobilized F(ab")2 fragments selectively bind CD5 molecules present in rabbit appendix cell lysates.

    Conclusions:

    • Interactions between VH framework region structures and CD5 may influence the maintenance and selective expansion of specific B cell populations.
    • These interactions could potentially contribute to the autostimulatory growth observed in autoimmune diseases or transformed cells.