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Transforming growth factor beta in bovine placentas
L Munson1, A Wilhite, V F Boltz
1Department of Pathology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
Transforming growth factor beta (TGF beta) are crucial for embryonic development. This study found TGF beta present in bovine placentomes, suggesting a role in promoting fetal and maternal tissue growth.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Cell Biology
Background:
- Transforming growth factor beta (TGF beta) are key embryonic morphogens.
- TGF beta may influence the development of epitheliochorial placentas.
Purpose of the Study:
- Investigate TGF beta localization, expression, secretion, and effects in bovine placentomes.
- Examine TGF beta roles in chorionic and endometrial epithelial cells.
Main Methods:
- Immunohistochemistry and Northern slot-blots for TGF beta localization and expression in placentomes.
- DNA synthesis assays and bioassays to assess TGF beta effects and secretion by cultured cells.
Main Results:
- All TGF beta forms (1, 2, 3) were immunolocalized in bovine placentomes.
- TGF beta mRNA expressed in chorioallantois, caruncles, and cultured epithelial cells.
- Both endometrial and trophoblastic cells secreted TGF beta and showed transient proliferation in response to it.
Conclusions:
- TGF beta are present at the fetal-maternal interface in bovine placentomes.
- TGF beta may play a role in promoting endometrial and chorionic growth during pregnancy.
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