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Transforming growth factor beta in bovine placentas
L Munson1, A Wilhite, V F Boltz
1Department of Pathology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
Biology of Reproduction
|October 1, 1996
Summary
Transforming growth factor beta (TGF beta) are crucial for embryonic development. This study found TGF beta present in bovine placentomes, suggesting a role in promoting fetal and maternal tissue growth.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Cell Biology
Background:
- Transforming growth factor beta (TGF beta) are key embryonic morphogens.
- TGF beta may influence the development of epitheliochorial placentas.
Purpose of the Study:
- Investigate TGF beta localization, expression, secretion, and effects in bovine placentomes.
- Examine TGF beta roles in chorionic and endometrial epithelial cells.
Main Methods:
- Immunohistochemistry and Northern slot-blots for TGF beta localization and expression in placentomes.
- DNA synthesis assays and bioassays to assess TGF beta effects and secretion by cultured cells.
Main Results:
- All TGF beta forms (1, 2, 3) were immunolocalized in bovine placentomes.
- TGF beta mRNA expressed in chorioallantois, caruncles, and cultured epithelial cells.
- Both endometrial and trophoblastic cells secreted TGF beta and showed transient proliferation in response to it.
Conclusions:
- TGF beta are present at the fetal-maternal interface in bovine placentomes.
- TGF beta may play a role in promoting endometrial and chorionic growth during pregnancy.