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Related Experiment Videos

ATP release and its prejunctional modulation

K Starke1, I von Kügelgen, B Driessen

  • 1Pharmakologisches Institut, Albert Ludwigs-Universität Freiburg, Freiburg im Breisgau, Germany.

Ciba Foundation Symposium
|January 1, 1996
PubMed
Summary

Sympathetic co-transmitters noradrenaline and ATP exhibit differential release and modulation. Prejunctional receptors fine-tune the release of each transmitter, impacting sympathetic neurotransmission.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Physiology

Background:

  • Sympathetic nerves release noradrenaline and adenosine triphosphate (ATP) as co-transmitters.
  • Understanding the regulation of co-transmitter release is crucial for autonomic nervous system function.

Purpose of the Study:

  • To investigate the release characteristics and modulation of noradrenaline and ATP from sympathetic nerves.
  • To identify prejunctional receptors involved in regulating the release of these co-transmitters.

Main Methods:

  • Electrical stimulation of isolated sympathetically innervated tissues.
  • Measurement of [3H]noradrenaline overflow and enzymic determination of ATP release.
  • Pharmacological blockade of ectonucleotidases, adrenoceptors, and purinoceptors.

Main Results:

  • ATP overflow was significantly increased by ectonucleotidase inhibition, indicating substantial post-release metabolism.
  • Neural release of ATP and [3H]noradrenaline was similarly reduced by prostaglandin E2.
  • Prejunctional alpha 2-adrenoceptors preferentially inhibited noradrenaline release, while A1 purinoceptors preferentially inhibited ATP release.
  • ATP inhibited [3H]noradrenaline release via both A1 and P2 purinoceptors, with P2 antagonists increasing noradrenaline release.

Conclusions:

  • Sympathetic co-transmitters noradrenaline and ATP are differentially stored, released, and modulated.
  • Postganglionic sympathetic axons express alpha 2 and P2 autoreceptors that regulate co-transmitter release.

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