Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computational simulations of stem-cell factor/c-kit receptor interaction

M C Menziani1, F Fanelli, P G De Benedetti

  • 1Dipartimento di Chimica, Università di Modena, Italy.

Proteins
|September 1, 1996
PubMed
Summary

This study reveals key molecular details of stem-cell factor (SCF) and its receptor c-Kit interactions. Understanding these binding mechanisms is crucial for hematopoiesis research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Placenta accreta spectrum: clinical and radiological follow-up after conservative management and maternal complications.

European journal of obstetrics, gynecology, and reproductive biology·2026
Same author

Sarcopenic obesity and reduced BMD in young men living with HIV: body composition and sex steroids interplay.

Journal of endocrinological investigation·2024
Same author

Congenital toxoplasmosis and audiological outcome: from a case series to a suggestion of patient-based schedule.

Frontiers in pediatrics·2024
Same author

New insights into the comorbid conditions of Turner syndrome: results from a long-term monocentric cohort study.

Journal of endocrinological investigation·2022
Same author

A closer look to the new frontier of artificial intelligence in the percutaneous treatment of primary lesions of the liver.

Medical oncology (Northwood, London, England)·2020
Same author

Steroid biomarkers for identifying non-classic adrenal hyperplasia due to 21-hydroxylase deficiency in a population of PCOS with suspicious levels of 17OH-progesterone.

Journal of endocrinological investigation·2020

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Stem-cell factor (SCF) is a critical cytokine for early hematopoiesis.
  • SCF exerts its function by binding to the c-Kit receptor tyrosine kinase.
  • Understanding SCF/c-Kit interactions is vital for hematopoiesis research.

Purpose of the Study:

  • To elucidate the molecular mechanisms of species-specific stem-cell factor (SCF) and c-Kit receptor interactions.
  • To identify structural determinants governing SCF quaternary structure stabilization and ligand recognition.

Main Methods:

  • Homology-based molecular modeling.
  • Computational simulations.
  • Analysis of conformational, topographical, and dynamic features of SCF and SCF/c-Kit complexes.

Related Experiment Videos

Main Results:

  • Structural models of human and rat SCF ligands were generated using M-CSF as a template.
  • Ligand-binding domains of the c-Kit receptor were modeled based on human Fc fragment.
  • Key factors stabilizing SCF structure and molecular determinants for c-Kit recognition were identified.

Conclusions:

  • The study provides detailed insights into the structural basis of SCF/c-Kit interactions.
  • Identified molecular determinants are crucial for understanding SCF function in hematopoiesis.
  • These findings can inform future therapeutic strategies targeting the SCF/c-Kit pathway.